Fig. 6: Comparison of normal and wasp infested lymph glands.
From: Single-cell transcriptome maps of myeloid blood cell lineages in Drosophila
a Lymph gland (Antp-gal4 UAS-GTRACE; Antp, magenta) remains at 24 h post infestation (PI) (DAPI, blue). Hemocytes disintegrate by 48 h PI. Graph indicates the number of DAPI+ cells at 24 h PI. Normal (n = 51), 24 h PI (n = 44). Mann-Whitney test, **P = 0.0011. b UMAP projections of major cell types defined in normal (top, ‘n’) and wasp infested (bottom, ‘i’) lymph glands at 96 h AEL (thus, 24 h PI). c Relative proportion (top) or normalized cell counts (bottom) of major cell types in normal (blue) and wasp infested (red) lymph glands. d Two-dimensional projections of subclustered cells in normal (left) and wasp infested (right) lymph glands. e Normalized cell counts (top) or proportional ratio (bottom) of subclustered cells in normal (blue) or wasp infested (red) lymph glands. f. Wasp infestation reduces STAT92EAct (top; green) or Dl+ (bottom; magenta) PH1 populations. Graphs represent quantitation of the number of STAT92E Act (top) or the proportion of Dl+ (bottom) cells after infestation (24 h PI). Normal (n = 27) or 24 h PI (n = 18) for Top and Normal (n = 10), or 24 h PI (n = 13). Mann–Whitney test, ***P < 0.0001. g Top 20 genes in iLM1 or iLM2. Color bar indicates the level of scaled gene expression. h Dot plot expresses the level of marker genes in normal (blue) or wasp infested (red) lymph glands (top) using Wilcoxon Rank-Sum test. Scaled expressions of cell-cycle regulating genes in subclusters upon wasp infestation (bottom). Dot color: average expression levels. Dot size: percentage of cells expressing cell cycle genes in a subcluster. Cell count for each subcluster is listed in Supplementary Table 5. i PSC cell numbers do not change upon wasp infestation by 24 h PI (Antp, magenta; Traced, white; Antp-gal4 UAS-GTRACE). Graph indicates the number of Antp+ cells. Normal (n = 19) or infested (n = 17). Mann–Whitney test, n.s.: not significant. White scale bar, 30 μm; Yellow scale bar, 3 μm. Lymph glands are demarcated by white dotted lines. Median value is shown in graphs. Colors in b and d indicate each cell type. Graphs indicate the number of cells in one lymph gland lobe.
