Fig. 1: Transcriptional expression and genomic context of lysozyme homolog-encoding bd0314. | Nature Communications

Fig. 1: Transcriptional expression and genomic context of lysozyme homolog-encoding bd0314.

From: A lysozyme with altered substrate specificity facilitates prey cell exit by the periplasmic predator Bdellovibrio bacteriovorus

Fig. 1

a Scheme of the genomic context of bd0314 including surrounding genes predicted to encode a benzoate membrane transport protein (benE), a glucose-inhibited division protein B (gidB), a thiol peroxidase (tpx), an ATP-dependent RNA helicase (rhlE), and two hypothetical proteins (bd0317, bd0318). b Transcriptional analysis by reverse transcriptase PCR showing the expression of lysozyme gene homolog bd0314 and housekeeping gene dnaK, during the host-dependent lifecycle of B. bacteriovorus HD100 as depicted in drawings above the agarose gel. AP: attack phase HD100 cells, 15–240: timepoint in minutes after the invasion, Ec: E. coli S17-1 prey cells, −: negative control with H2O, +: positive control with genomic DNA of B. bacteriovorous HD100, L: 100 bp DNA ladder. This figure shows one of two biological repeats with similar results.

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