Fig. 5: Exogenously added lysozyme DslA releases B. bacteriovorus from 1 h bdelloplast. | Nature Communications

Fig. 5: Exogenously added lysozyme DslA releases B. bacteriovorus from 1 h bdelloplast.

From: A lysozyme with altered substrate specificity facilitates prey cell exit by the periplasmic predator Bdellovibrio bacteriovorus

Fig. 5

a Experimental scheme including different stains/fluorescent proteins to enhance visualization of different compartments. The experimental outline is described in the legend of Supplementary Fig. 8. b Example images of different outcomes after treatment copied from Supplementary Fig. 8. B. bacteriovorus bd0064:mcherry is shown in magenta, prey peptidoglycan in blue. Scale bar is 2 μm. c Percentage of bdelloplasts categorized according to the position of the Bdellovibrio in respect to the bdelloplast after treatment as described above (Supplementary Fig. 8). Individual symbols represent % of all categorized bdelloplasts per experiment. A minimum of 71 bdelloplasts per treatment condition and biological repeat were evaluated (for details please refer to Source Data file). The number of biological repeats is indicated in the legend as n for the respective treatment. Horizontal lines represent median, statistical test was Mann–Whitney, ns non-significant, p-values = 0.004 (all p-values are one-tailed). HEWL hen egg-white lysozyme.

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