Fig. 1: Graphical outline of the experimental design and sample collection.
From: Ocular conjunctival inoculation of SARS-CoV-2 can cause mild COVID-19 in rhesus macaques
Five male rhesus macaques (Macaca mulatta) were inoculated with 106 TCID50/mL SARS-CoV-2. Two rhesus macaques were inoculated via the ocular conjunctival route and named CJ-1 and CJ-2, one was inoculated via the intratracheal route and named IT-1, and two were inoculated via the intragastric route in sequence and named IG-1 and IG-2. The macaques were observed for clinical signs (body weight and temperature were tested as shown). On 0, 1, 3, 5, and 7 dpi, conjunctival, nasal, throat, and anal swabs were collected. CJ-1, IT-1, and IG-1 were euthanized and necropsied at 7 dpi. Tissues were collected to analyse the viral loads and titres. Serum was collected from all animals at 0 and 7 dpi and from CJ-2 and IG-2 at 14 and 21 dpi to examine the specific IgG antibodies against SARS-CoV-2 antigens.
