Fig. 1: Innate T cells have analogous effector subsets.
From: Single-cell RNA sequencing identifies shared differentiation paths of mouse thymic innate T cells
a Single-cell suspensions of 7-week-old BALB/c thymocytes were stained with PBS57 loaded CD1d tetramer, 5-OP-RU loaded MR1 tetramer, and anti-TCRγδ (GL3), and enriched for iNKT, MAIT, and γδ T cells respectively using MACS beads. Representative dot plots are shown and numbers indicate frequencies of cells in adjacent gates. Representative results of at least 10 independent experiments are shown. b Heat map shows log2 values of mean fluorescence intensities of cells expressing indicated markers analyzed by flow cytometry. Hierarchical clustering was made by Pearson correlation. Representative results of three independent experiments are shown. c Heat maps show expression patterns of cytokines, receptors, and transcription factors mapped to overexpressed genes in each Tγδ subset. Expression patterns were quantified by column Z-scores of regularized log2-value of read counts. d Principal component analysis (PCA) plot shows subset distribution of Tγδ subsets. Each dot represents a biological replicate. e PCA plot using 120 functional genes including cytokines, receptors, and transcription factors shows subset distribution of iNKT and γδ T cells. Each dot represents a biological replicate. f MAIT cells were enriched from Tcrd KO mice and analyzed for their subset profiles. Graph shows statistical analysis of number of MAIT subsets in indicated mice (n = 5 except analysis for CD24hi stage 1 MAIT cells (n = 3), right). Results are pooled from three independent experiment and numbers indicate frequencies of cells in adjacent gates (left). Each dot represents an individual mouse. Data are presented as mean ± SD. Unpaired two-tailed t-test was used. *P < 0.05, **P < 0.01. Source data are provided as a Source Data file.
