Fig. 7: Human and mouse innate T cells have analogous subsets.
From: Single-cell RNA sequencing identifies shared differentiation paths of mouse thymic innate T cells
a Human mononuclear cells obtained from liver perfusion fluid were stained with the indicated markers. Representative dot plots (left) show frequencies of iNKT (left), MAIT (middle), and γδ T cells (right) amongst total mononuclear cells. Representative FACS plots are from seven independent experiments and graph shows statistical analysis of their frequencies using pooled data (n = 4 for NKT cell analysis, n = 6 for MAIT cell analysis, n = 4 for γδ T cell analysis). b Total γδ T cells from (a) were stained with the indicated anti-TCR antibodies and representative FACS plots are from three independent experiments (left) and graph shows pooled results of their frequencies with statistical analysis (right, n = 5 except Vδ2+Vγ9+ cell analysis (n = 4)). c Indicated cells were stimulated with PMA and ionomycin, and intracellularly stained with the anti-cytokine antibodies. Representative dot plots are shown from three independent experiments. Numbers indicated frequencies of cells in adjacent gates or each quadrant. Data are presented as mean ± SD. Unpaired two-tailed t-test was used. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. NS not significant (P > 0.05). Source data are provided as a Source Data file.
