Fig. 2: Diameters of myelinated axons are abnormally increased when CMTM6 is lacking from Schwann cells.
From: CMTM6 expressed on the adaxonal Schwann cell surface restricts axonal diameters in peripheral nerves
a Electron micrographs of cross-sectioned phrenic nerves reveal increased axonal diameters in Cmtm6-cKo compared to control mice at 2 months (2 mo). Scale bar, 5 µm. b Genotype-dependent assessment of myelinated axons on semi-thin sections confirms shift towards larger axonal diameters in phrenic nerves of Cmtm6-cKO compared to control mice. Data are presented as frequency distribution with 0.5 µm bin width. n = 1293 axons from n = 5 control mice and n = 1172 axons from n = 5 Cmtm6-cKO mice; Mean axonal diameter (control+/−Cmtm6-cKo) = 2.87 µm + 0.84 µm; P = 2.2e−16 by two-sided Kolmogorow–Smirnow test of frequency distributions. For sciatic nerves and tail nerves, Supplementary Fig. 5; g-ratios Supplementary Fig. 6; nodal parameters Supplementary Fig. 7; neurofilament density Supplementary Fig. 8; tamoxifen-induced deletion in adult mice Fig. 4. c Genotype-dependent quantification on semi-thin sections shows unchanged number of myelinated axons in the phrenic nerves of Cmtm6-cKo-mice. n = 5 mice per genotype; P = 0.1929 by two-tailed Student’s t-test. d Representative electron micrographs of cross-sectioned dorsal roots show increased axonal diameters in Cmtm6-cKo compared to control mice at 2 mo. Scale bar 5 µm. e Genotype-dependent assessment of the diameters of myelinated axons on semi-thin sections confirms the shift towards larger axonal diameters in dorsal roots of Cmtm6-cKO-mice. Data are presented as frequency distribution with 0.5 µm bin width, n = 10387 axons from n = 5 control mice and n = 9298 axons from n = 5 Cmtm6-cKO mice; mean axonal diameter (control+/−Cmtm6-cKo) = 2.47 µm + 0.27 µm; P = 2.2e−16 by two-sided Kolmogorow–Smirnow test of frequency distributions. f Genotype-dependent quantification on semi-thin sections shows unchanged numbers of myelinated axons in the dorsal root of Cmtm6-cKo-mice. n = 5 mice per genotype; P = 0.3894 by two-tailed Student’s t-test. Data in c and f presented as mean ± SD. n.s. = non-significant P > 0.05; ***P < 0.001. Source data see Source Data file.
