Fig. 4: Adult deletion of Cmtm6 causes increased axonal diameters.
From: CMTM6 expressed on the adaxonal Schwann cell surface restricts axonal diameters in peripheral nerves
a Scheme for tamoxifen injection and time points post tamoxifen injection (PTI) for analyzing control mice (genotype Cmtm6flox/flox) and Cmtm6-iKo-mice (genotype Cmtm6flox/flox;PlpCreERT2). b, c Representative electron micrographs of cross-sectioned sciatic nerves show increased axonal diameters in Cmtm6-iKo compared to control mice 2 mo PTI (b) and 6 mo PTI (c). Scale bar, 2.5 µm. d, e Genotype-dependent quantification of diameters of myelinated axons in sciatic nerves dissected from Cmtm6-iKo and control mice 2 mo PTI (d) or 6 mo PTI (e). Note the frequency shift toward increased axonal diameters in Cmtm6-iKo-mice at both time points PTI. Data are presented as frequency distribution with 0.5 µm bin width; (d) n = 14814 axons from n = 4 control mice and n = 20067 axons from n = 5 Cmtm6-iKO mice; Mean axonal diameter (control+/−Cmtm6-iKo) = 3.57 µm + 0.03 µm; P = 0.002 by two-sided Kolmogorow–Smirnow test; e n = 19,025 axons from n = 5 control mice and n = 15,047 axons from n = 4 Cmtm6-iKO mice; mean axonal diameter (control+/−Cmtm6-iKo) = 4.05 µm + 0.17 µm; P = 2.98e−10 by two-sided Kolmogorow–Smirnow test of frequency distributions. f, g Quantifications on semi-thin sections shows normal number of myelinated axons in Cmtm6-iKo compared to control mice 2 mo PTI (f) and 6 mo PTI (g). f n = 4 control and n = 5 Cmtm6-iKo mice, P = 0.2771 (g) n = 5 control and n = 4 Cmtm6-iKo mice, P = 0.5394; by two-tailed Student’s t-test. Data in (f) and (g) presented as mean ± SD. n.s. = non-significant *P > 0.05; **P < 0.01; ***P < 0.001. Source data see Source Data file.
