Fig. 1: Nanobody discovery.
From: An alpaca nanobody neutralizes SARS-CoV-2 by blocking receptor interaction
a Overview of the nanobody generation process. b Variant frequencies quantified by next generation sequencing (NGS) across successive enrichment steps. Identified using receptor-binding domain (RBD) bait, Ty1 exhibits the greatest total fold change of all nanobodies, increasing in proportion over 10,000-fold between initial and final libraries. c Sequence of Ty1. Complementarity-determining regions (CDRs) are indicated. Amino acid color labels; hydrophobic, blue; positive charge, red; negative charge, magenta; aromatic, cyan; polar, green; cysteine, pink; glycine, orange; proline, yellow.
