Table 1 Validation of library-enriched HLA-DR401-binding motif. Table of peptides either enriched by our randomized 9mer HLA-DR401 library selections, but not predicted to bind HLA-DR401 by NetMHCII 2.3 or IEDB Consensus, or derived from Influenza A virus and predicted to bind HLA-DR401 but not matching our enriched motif, with algorithmic prediction values and IC50 values measured via fluorescence polarization competition assays.
Peptide | Measured IC50 (nM) | NetMHCII 2.3 IC50 (nM) | IEDB Consensus Rank (%) | TEPITOPE Rank (%) | NeonMHC2 Rank (%) | NetMHCIIpan 4.0 EL Rank (%) |
|---|---|---|---|---|---|---|
AAANMDTSLPAWEEG | 180 | 1922 | 48 | 86 | 34 | 25 |
AAERKMSVLSAWEEG | 817 | 2444 | 54 | 85 | 28 | 30 |
AAGVIDPTMLGWEEG | 29 | 1378 | 66 | 91 | 25 | 24 |
AALNVERTCHCWEEG | 33 | 13,045 | 95 | 2.2 | 7.5 | 28 |
AALREEHTCKCWEEG | 37 | 8801 | 88 | 3.2 | 4.5 | 29 |
AALSLERSCKCWEEG | 25 | 8192 | 87 | 12 | 3.6 | 52 |
AALVDDPTCRCWEEG | 29 | 6089 | 79 | 4.7 | 6.9 | 18 |
AAVADDFSCRGWEEG | 47 | 6836 | 82 | 60 | 27 | 34 |
AAWDPDKTVYGWEEG | 44 | 1866 | 47 | 22 | 0.5 | 0.6 |
AAWDPERTCRAWEEG | 32 | 5921 | 79 | 9.5 | 0.7 | 11 |
AAWERENDMLGWEEG | 15 | 1480 | 42 | 28 | 0.9 | 1.9 |
AAWESSTDLVGWEEG | 12 | 1365 | 50 | 50 | 13.7 | 4.9 |
AAWHGEGSQIGWEEG | 18 | 1728 | 45 | 8.8 | 0.3 | 3.2 |
AAWHNDPACKGWEEG | 41 | 12,112 | 93 | 17 | 1.1 | 6.0 |
AAWVPCGDMVSWEEG | 26 | 4439 | 71 | 6.3 | 7.3 | 13 |
AAWVVEHSEVGWEEG | 19 | 1345 | 39 | 0.9 | 0.2 | 0.5 |
KGYMFESKSMKLRTQ | 38,661 | 138 | 16 | 36 | 40 | 28 |
LFEKFFPSSSYRRPV |  > 50,000 | 172 | 11 | 32 | 9.5 | 15 |
NQNIITYKNSTWVKD | 43,436 | 75 | 3.8 | 49 | 45 | 20 |
SFFYRYGFVANFSME |  > 50,000 | 35 | 6.1 | 24 | 18 | 31 |
SRMQFSSFTVNVRGS | 3,381 | 81 | 6.7 | 54 | 14 | 12 |
VSSFQDILLRMSKMQ |  > 50,000 | 101 | 9.4 | 39 | 42 | 50 |
VVNFVSMEFSLTDPR | 7969 | 34 | 5.5 | 19 | 8 | 17 |
YWKQWLSLRNPILVF | 2515 | 30 | 1.7 | 19 | 2.7 | 15 |
