Fig. 1: Overview of generation of the hypothalamus scRNA-seq dataset.

a UMAP plot showing scRNA-seq data obtained from the developing diencephalon (including the prethalamus and hypothalamus) between E10 and E16, E18, P4, P8, P14, and P45. RNA velocity marks neuronal or glial (oligodendrocytes, astrocytes, ependymal cells, and tanycytes) trajectories. b UMAP plot showing neuronal clusters across the entire course of hypothalamus development. c Heatmap showing subtypes of neuronal clusters based on neuropeptide and transcription factor expression.