Fig. 3: Utilizing HyDD to infer the identity and origin of individual cell types.
a The HyDD dataset was used to train a previously published scRNA-seq on E15.5 hypothalamus obtained through selective dissection of Pomc-EGFP-expressing cells53. b Alluvial plot showing HyDD clusters (left) matched to clusters from Huisman et al.53 (right). Note that 2 clusters (clusters 2 and 4) from Huisman et al.53 do not match the HyDD dataset. c Using the molecular stepping stone approach to identify VMH neurons (green) across the entire developmental ages by identification of shared sets of gene modules that can demarcate the VMH across the entire hypothalamus scRNA-seq dataset. d HyDD dataset is used to identify the developmental origins of previously annotated subtypes of glutamatergic neurons of the core VMH55 (top), and to identify the developmental origins of GABAergic neurons surrounding the core VMH (bottom).
