Fig. 4: scRNA-seq-based analysis of Nkx2-1-deficient developing hypothalamus. | Nature Communications

Fig. 4: scRNA-seq-based analysis of Nkx2-1-deficient developing hypothalamus.

From: The cellular and molecular landscape of hypothalamic patterning and differentiation from embryonic to late postnatal development

Fig. 4

a UMAP plot showing clusters from combined scRNA-seq dataset of control (Nkx2-1CreER/+) and Nkx2-1 mutant line (Nkx2-1CreER/CreER), in which clusters were obtained by training the dataset using HyDD markers. b UMAP heatmap plot showing distribution of individual clusters between control (left) and Nkx2-1 mutants (right). c Bar graph showing the distribution of individual clusters between control and Nkx2-1 mutants. d Jitter plots of regional marker genes (Chchd10 and Sox14—ARC/VMH, Hmx2 and Hmx3—PMN, Foxb1 and Lhx1—MMN between control and Nkx2-1 mutants. e Schematic showing overall hypothalamic phenotype between control and Nkx2-1 mutants. Note the absence of ventral diencephalic structures (except the supramammillary nucleus), and the relative expansion of the prethalamus in Nkx2-1 mutants (right).

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