Fig. 3: 7E decreased macrophage infiltration in an orthotopic model of pancreatic cancer.
From: IL-20 antagonist suppresses PD-L1 expression and prolongs survival in pancreatic cancer models
KPC cells were injected into the pancreas of mice. PBS, mIgG, or 7E treatment was performed (n = 5), and tumors were harvested at day 28. a IHC staining for F4/80 (one-way ANOVA, p = 0.0131) and CD206 (one-way ANOVA, p < 0.0001) counterstaining in a tumor tissue sample. Immunohistochemical quantification with the HistoQuest software (right) demonstrated a reduction in infiltrating macrophage numbers in the tumor (n = 5). Original magnification: ×200; scale bar, 50 μm. The rightmost panel shows the IgG-stained negative control. ***p < 0.001 compared with the mIgG-treated controls. b Flowchart of treatment. BMDMs were treated with IL-20 for 48 h, and 7E was used to neutralize the activity of IL-20. c The number of CD206 (one-way ANOVA, p = 0.0003) and CD86 (one-way ANOVA, p = 0.0089) in macrophages was determined by flow cytometry. The results in the bar graph (right) represent the average value of three independent experiments. *p < 0.05, **p < 0.01. Data are presented as mean ± SD. Statistical significance was determined by one-way ANOVA Sidak’s multiple comparisons test (a, c).
