Fig. 5: 7E reversed fat mass loss in an orthotopic model of pancreatic cancer.

KPC/Luc cells were injected into the pancreas of mice. PBS, mIgG, or 7E (n = 5) was injected (i.p.) into the mice twice per week for 28 days. Mice not injected with cancer cells served as healthy controls (n = 3). a The tumor-free body weight of the mice was measured on day 28. One-way ANOVA, p < 0.0001; ****p < 0.0001. b The food intake of the mice was monitored every week. Data are shown as the mean. c Epididymal fat was excised and weighed. One-way ANOVA, p < 0.0001; ****p < 0.0001. d IHC staining for F4/80+ macrophages in the epididymal fat of orthotopic model mice was performed. Original magnification: ×400; scale bar, 10 μm. e Representative images of epididymal fat sections stained with H&E for histological analysis are shown. Original magnification: ×200; scale bar, 100 μm. f Adipocyte size was quantified from randomly selected 10 cells (n = 10 cells examined). One-way ANOVA, p < 0.0001; ***p < 0.001; ****p < 0.0001. g IHC staining for DAG-1 in the soleus muscle of orthotopic model mice was performed. Original magnification: ×200; scale bar, 50 μm. Values represent the mean ± SD. Statistical significance was determined by one-way ANOVA Sidak’s multiple comparisons test (a, c, f).