Fig. 11: Immunofluorescence staining of neuron cells and axons.

a Immunofluorescence staining of neuron cells (scale bars are 50 µm, 3 independent experiments) and b quantification analyzed with optical density (n = 5, Tuj-1 intensity values were mean ± std. dev., ^%p < 0.0001 when comparing blank control and microsol electrospun groups (MSaP, MSaP-aL, and MSaP-aL/p); ^&p < 0.05 when comparing aP and microsol electrospun groups (MSaP, MSaP-aL, and MSaP-aL/p); ^@p < 0.01 when comparing 4w and 8w in MSaP; ^$p < 0.01 when comparing 4w and 8w in MSaP-aL and ^#p < 0.01 when comparing 4w and 8w in MSaP-aL/p; **p < 0.01, ****p < 0.0001 when comparing MSaP-aL/p and other control groups at the same time point via two-way analysis of variance (ANOVA) with Tukey’s post hoc test). c Immunofluorescence staining of axon (scale bars were 50 µm, 3 independent experiments) with d its quantification analyzed with optical density (n = 5, GAP-43 intensity values were mean ± std. dev., ^#p < 0.05 when comparing 4w and 8w in blank control, aP, MSaP, MSaP-aL, and MSaP-aL/p, respectively; ^@p < 0.01 when comparing sham group and other groups, ^&p < 0.0001 when comparing aP and microsol electrospun groups (MSaP, MSaP-aL, and MSaP-aL/p), and ***p < 0.001, ****p < 0.0001 when comparing MSaP-aL/p and other control groups at the same time point via two-way analysis of variance (ANOVA) with Tukey’s post hoc test).