Fig. 7: Neuron-specific marker expression.

a Immunofluorescence staining of Tuj-1, NSE, NF-200, and Tau staining images on different fiber scaffolds (scale bars were 100 µm, 4 independent experiments) with b–e corresponding fluorescence semi-quantitative analysis. f–i mRNA expression analysis of Tuj-1, NSE, NF-200, and Tau. The yellow bi-directional arrows represented the contact guiding effect of oriented nanofibers on stem cells (n = 3, all values were mean ± std. dev., **p < 0.01, ***p < 0.001, and ****p < 0.0001 when comparing microsol electrospun groups (MSaP, MSaP-aL, MSaP-aL/p) and control and aP groups, respectively, via one-way analysis of variance (ANOVA) with Tukey’s post hoc test, ns not significant).