Fig. 3: MI promotes health in middle-aged mice.

a Design of mouse experiment. b, c GSEA results show mouse aging-downregulated or upregulated gene expressions, measured by RNA-seq log₂-fold-change in FPKM, were reversely regulated by MI supplementary (GSEA p < 0.001 and p = 0.0701 respectively), N = 1. d MI increased mouse running distance in 20 min (Data are presented as mean values ± SEM, n = 6, two-sided t-test *p < 0.05, p = 0.028 between CTL and MI), N = 2. e MI increased mouse limbs grip strength (Data are presented as mean values ± SEM, n = 6, two-sided t-test *p < 0.05, p = 0.0001 between CTL and MI), N = 2. f MI decreased mouse body fat content (Data are presented as mean values ± SEM, n = 8 for CTL, n = 4 for others, two-sided t-test n.s. not significant, p = 0.12 between CTL and MI), N = 1. g Clustering of the aging-related genes, which were reversed by MI. Cluster 0 and 1 are aging downregulated genes, that were upregulated by MI, cluster 4 and 5 are aging upregulated genes, that were downregulated by MI. Cluster 2 or 3 show genes, which didn’t change obviously from M3 to M12, but were downregulated or upregulated by MI, respectively. Source data are provided as a Source Data file and Supplementary Dataset 1.