Fig. 5: The aging-alleviating effect of MI completely depends on DAF-18(PTEN).
From: The precursor of PI(3,4,5)P3 alleviates aging by activating daf-18(Pten) and independent of daf-16
a daf-18-NL [daf-18(ok480)] fully blocked the longevity effect of MI, N = 4. b daf-18-NL fully blocked inos-1 OE longevity effect, N = 2. c, d MI supplementation could not reverse the aging-up or downregulated genes expression changes in daf-18-NL worms (GSEA p < 0.0001 for both), N = 1. e MI could not increase the mobility of old (AD_8) daf-18-NL worms as in control N2 worms (n = 10 for each group, two-sided t-test n.s. not significant), N = 2. f MI could not inhibit the fat storage in old (AD_8) daf-18-NL worms as in control N2 worms (n = 30 in each group, two-sided t-test n.s. not significant), N = 3. g MI supplementation upregulated the PTEN protein level in mouse muscle, N = 3. h Quantification of the immunoblotting result (two-sided t-test p = 0.1). Source data are provided as a Source Data file.
