Fig. 1: Generation of a wrapping glia driver. | Nature Communications

Fig. 1: Generation of a wrapping glia driver.

From: Wrapping glia regulates neuronal signaling speed and precision in the peripheral nervous system of Drosophila

Fig. 1

Confocal projection of larval filet preparations of the genotypes indicated. Representative images are shown taken from >10 animals analyzed for each genotype. a Third instar larva with the genotype [nrv2-stGFP]. Broad GFP expression is detected in the CNS. Note the restricted expression in the peripheral nervous system which corresponds to the wrapping glia (arrowheads). b Third instar larva of the genotype [hs-Flp; nrv2-Gal4/MCFO-2]. flp expression of the multicolor FlpOut construct was induced by 1 h 37 °C heat shock during first instar stage. Larvae were stained for HA (green), V5 (red), and FLAG (blue). c Same animal as in (a). Expression of stRed is observed only in the CNS and no expression is found in the wrapping glia. d–f Overlay of nrv2-GFP (green) and 90C03 > dsRed (red) expression. Note the complete overlap of dsRed (e) and GFP expression in the CNS (f). g Young third instar larva with the genotype [hs-Flp; nrv2-Gal4/MCFO-2; 90C03-Gal80]. flp expression was induced by 1 h 37 °C heat shock during first instar stage. Larvae were stained for HA (green), V5 (red), and FLAG (blue). h Living third instar larva of the genotype [nrv2-Gal4, UAS-CD8GFP; 90C03-Gal80/90C03-Gal80]. Note strong expression at the anterior tip of the larva. Scale bars are 250 µm (a–c, g, h) and 100 µm (d–f).

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