Fig. 10: Modulation of the Goro circuit. | Nature Communications

Fig. 10: Modulation of the Goro circuit.

From: Wrapping glia regulates neuronal signaling speed and precision in the peripheral nervous system of Drosophila

Fig. 10

a Upon optogenetic activation of the mdIV neurons in third instar control larvae [ppk-LexA, nrv2-Gal4/UAS-GFP; LexAop-csChrimson, nrv2-Gal4/90C03-Gal80] rolling is induced after 210 ms (n = 38 larvae). Upon ablation of the wrapping glia in third instar larvae [ppk-LexA, nrv2-Gal4/UAS-hid; LexAop-csChrimson, nrv2-Gal4/90C03-Gal80] no rolling response is induced but larvae cramp after 80 ms (n = 28 larvae; p = 7E−10). Upon expression of htlDN [ppk-LexA, nrv2-Gal4/UAS-htlDN; LexAop-csChrimson, nrv2-Gal4/90C03-Gal80] a rolling response is initiated as in control but with a delay of 120 ms (n = 32 larvae, p = 1.9E−5). b In control larvae optogenetic activation of the Goro neurons [Goro-LexA/UAS-GFP; LexAop-csChrimson, nrv2-Gal4/90C03-Gal80] induces rolling after 475 ms (n = 27 larvae). Upon ablation of wrapping glia [Goro-LexA/UAS-hid; LexAop-csChrimson, nrv2-Gal4/90C03-Gal80] the rolling response is induced at about the same time (n = 43 larvae, p = 0.212). A slightly significant delay in the rolling response can be observed when comparing to larvae expressing htlDN [Goro-LexA, nrv2-Gal4/UAS-htlDN; LexAop-csChrimson, nrv2-Gal4/90C03-Gal80] (n = 26 larvae, p = 0.013). c Control larvae show 5 rolls (n = 23 larvae, median value). Upon ablation of wrapping glia 9 rolls are induced (median value, n = 33 larvae, p = 0.000074). Larvae expressing htlDN in wrapping glia show 6 rolls which is not significantly different from the control (n = 20 larvae, p = 0.48). Genotypes as in (b). d The orientation of rolling upon Goro activation changes when wrapping glia are ablated but not when wrapping glia differentiation is impaired. Control larvae (n = 27) and larvae expressing htlDN (n = 20) do not change orientation of rolling reaction (p = 0.43). In contrast upon wrapping glia ablation larvae change orientation during every other roll (n = 39 larvae, p = 0.005). Genotypes as in (b). Box plots: median (horizontal line), boxes represent first and third quartile, whiskers show standard deviation, individual points show outliers. All statistical analyses: t test.

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