Fig. 3: Wrapping glia affects neuronal differentiation and behavior.
a Distribution of axonal size in 40 nm2 bins of control larvae ([nrv2-Gal4, UAS-GFP; 90C03-Gal80/90C03-Gal80], 2046 axons of 26 nerves) and of larvae expressing htlDN in wrapping glia ([nrv2-Gal4/UAS-htlDN; 90C03-Gal80/90C03-Gal80]; 2555 axons of 22 nerves). The number of axons in the indicated size intervals is plotted for control (green) and htlDN nerves (blue). b Same dataset as in (a). Relative changes in the number of axons in different axon size classes upon expression of htlDN compared to control animals. Note the increase in the number of small diameter axons and the reduction of larger caliber axons. c The wrapping index of control larvae [white1118] and larvae expressing htlDN in the wrapping glia [nrv2-Gal4; UAS-htlDN]. The wrapping index in control nerves is 18.5 (n = 27 nerves). The wrapping index is 7.5 upon heartless suppression (n = 22 nerves, p = 3.5E−11, t test). d The accumulated distance per minute is not changed by expression of htlDN (genotypes as indicated). n = 68 control larvae and n = 71 larvae expressing htlDN, p = 0.85, Wilcoxon rank-sum test. Box plots in (c, d) show median (horizontal line), boxes represent the first and third quartile, whiskers show standard deviation, individual points show outliers. e Bending angle distribution of control larvae and larvae expressing htlDN in wrapping glia (genotypes as in (a)). A slight difference in the bending behavior can be detected (p = 8.4E–32, t test). f Quantification of the coiling phenotype. The relative distribution of 300 frames long movement clips (n = 263 30-s long video clips with 300 frames each) with 0, 1–30, or 31–60 frames showing coiling is shown. Green denotes control, blue denotes larvae expressing htlDN in wrapping glia, genotypes as in (a).
