Fig. 3: Caspase-8 activation in Pc-infected mice.

All results presented in this figure were obtained from mice either uninfected or at 8 days after infection with Pc. C57BL/6, Casp1/11^−/−, Ripk3^−/−/Casp8^−/−, Ripk3^−/−/Casp8/1/11^−/−, Ifng^−/−, and Tnfr^−/− mice were used in different experiments, as indicated. CD11b⁺ cells purified from splenocytes were lysed in RIPA buffer to prepare the lysates. Alternatively, splenocytes were spin down 400 × g for 5 min to prepare supernatants. Lysates from CD11b⁺ cells isolated from spleens of control and Pc-infected mice were analyzed by western blot using either a two distinct anti-caspase-8; b anti-caspase-1 and anti-caspase-11; c anti-caspase-1; d anti-caspase-11; and e anti-caspase-8 antibodies. b, d Alternatively, splenocytes supernatants were used to detect active caspase-11 (p30) by western blot using an anti-caspase-11 antibody. f Lysates of CD11b⁺ cells from uninfected and infected C57BL/6 and Tnfr^−/− mice were analyzed by western blot with anti-caspase-8, anti-caspase-1, and anti-caspase-11 antibodies. Blots revealed with anti-β-actin were used as loading controls. Blots are representative of two to three different experiments with similar results.