Fig. 7: Activation of caspases-4 and -8 in monocytes from malaria patients.

CD14⁺ monocytes were isolated from peripheral blood mononuclear cells (PBMCs) from healthy donors (HD) and malaria patients (P. vivax), lysed with RIPA buffer and analyzed by western blot. a Western blot was performed with an anti-caspase-4 antibody. The levels of caspase-4 p32 expression were analyzed by densitometry and shown in the bottom panel (HD n = 7 and P. vivax n = 8). b Western blot was performed with CD14⁺ monocytes lysates from two HD and five P. vivax malaria patients, and expression of caspase-8 (p55) and cleaved caspase-8 (p18) detected using an anti-caspase-8 antibody. The levels of caspase-8 p18 expression were analyzed by densitometry and shown in the bottom panel (HD n = 4 and P. vivax n = 9). c Western blot was performed with CD14⁺ monocytes lysates from HD as well as P. vivax malaria patients and revealed with an anti-GSDM-D antibody. The levels of cleaved GSDM-D p30 expression were analyzed by densitometry and shown in the bottom panel (HD n = 7 and P. vivax n = 9). d CD14 + monocytes lysate isolated from healthy donors (HD) and malaria patients (P. falciparum) were analyzed by western blot performed against caspase-8 and GSDM-D. The levels of caspase-8 and GSDM-D expression were analyzed by densitometry and shown in the side panels (HD n = 3 and P. falciparum n = 6). e CD14 + monocytes lysate isolated from healthy donors (HD) and malaria patients (P. falciparum) were analyzed by western blot performed against caspase-4. The levels of caspase-4 expression were analyzed by densitometry and shown in the bottom panel (HD n = 4 and P. falciparum n = 5). Statistical analysis was performed by two-tailed unpaired Mann–Whitney test at 95% CI. Densitometry graphs show mean ± s.e.m of samples from patients run on two to three representative gels with similar results.