Fig. 1: Rhythms in oxygen consumption and protein abundance at three dilution rates. | Nature Communications

Fig. 1: Rhythms in oxygen consumption and protein abundance at three dilution rates.

From: Eukaryotic cell biology is temporally coordinated to support the energetic demands of protein homeostasis

Fig. 1

a The period (τ) of oscillation, which is made up of stages of higher and lower O2 consumption (HOC, LOC), varies with dilution rate (mean ± SEM are used throughout, n = 4 biologically independent samples). Samples were harvested at the times shown. b the duration of LOC, not HOC, varies with dilution rate (extra sum-of-squares F test: straight line vs. horizontal line fit, pHOC = 0.07, n = 4 biologically independent samples). c Of 3389 proteins detected by quantitative mass spectrometry, only 4% were consistently rhythmic (varied by >33% across all conditions). d Heatmap showing mean abundance of consistently rhythmic proteins clustered with either LOC or HOC (see also Supplementary Data 1). e Oxygen consumption and mean-normalized protein abundance, for representative examples of HOC (high-affinity sulfate permease, Sul1) and LOC (monocarboxylate transporter, Jen1) phase proteins. Source data are provided as a Source Data file.

Back to article page