Fig. 4: YROs regulate stress granules and glycogen, and are sensitive to H+ and K+. | Nature Communications

Fig. 4: YROs regulate stress granules and glycogen, and are sensitive to H+ and K+.

From: Eukaryotic cell biology is temporally coordinated to support the energetic demands of protein homeostasis

Fig. 4

a The intensity and distribution (STD/mean) of stress granule marker Pab1 (Pab1-GFP signal) varies over the YRO, with more foci during LOC and more diffuse during HOC, supporting dynamic variation in stress granule formation (The scale bar represents 1 µm. OWA, nT90h = 4 images and nT92-97h = 8 images one experiment, n ≥ 72 granules per time point). b Cellular glycogen stores increase during LOC and decrease during HOC (OWA, n = 3 biological replicates). Liquidation of storage carbohydrates is likely to fuel translational bursting during HOC. c, d Decreasing extracellular pH reduces the period of the YRO duration (representative OCR, npH3.4 = 4 or n = 3 independent experiments). e, f HSP30 mutants have truncated oscillations (maroon/red, pHIC; black/grey, representative OCR, n = 4 biological replicates). g, h Extracellular K+ concentration determines YRO period duration (representative OCR). This is unlikely to be due to loss of cell viability as YROs are rapidly restored when potassium becomes available (representative OCR). All data are shown as mean ± SEM. Source data are provided as a Source Data file.

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