Fig. 5: Pro-survival Mcl-1 is critical for LUAD development.
From: MCL-1 gains occur with high frequency in lung adenocarcinoma and can be targeted therapeutically
a Experimental design. b, c Representative images (b) and quantification (c) of Mcl-1 mRNA in situ hybridisation in lesions and adjacent healthy tissue. Inserts are higher magnification of corresponding images. n = number of lesions. Data were analysed by one-way ANOVA (p = 6.600E−9, F(2,39) = 31.73, p values from post-hoc analysis with Bonferroni correction are reported in figure. d Representative H&E staining of lung tissue sections at 19 weeks after AdCre virus infection. Inserts are higher magnification of indicated areas. Scale bar represents 2 mm, 1 mm, 100 µm, or 10 µm. e Number of lesions per section. n = number of sections. Three sections per animal, separated by 100 µm, were assessed. Data were analysed by Kruskall–Wallis test (p = 5.544E–6, p values from post-hoc analysis with Dunn’s correction are reported in figure). f Representative renderings of the µ-CT analysis. g Quantification of tumour volume expressed as percentage of the lung volume. n = number of mice. Data were analysed by one-way ANOVA (p = 0.0145, F(2,10) = 6.656, p values from post-hoc analysis with Bonferroni correction are reported in figure). Data in panel (c, e, and g) are representative of four independent experiments and are presented as dot plot and show mean ± SEM. h Representative images of pulmospheres generated from dissected tumour tissue (2 lesions from 2 mice/genotype). Inserts showing individual pulmospheres. Scale bar is 25 µm or 100 µm. i Quantification of pulmosphere area (µm2) over the cultivation period. n = number of pulmospheres. Data are presented as mean ± SEM and were analysed by repeated measure two-way ANOVA (for time, genotype, and interaction effects, p < 0.0001). p values from post-hoc analysis with Bonferroni correction are reported in the panel (**p < 0.01 and ***p < 0.001).
