Fig. 2: Substrate degradations by asymmetric proteasomes.
From: Allosteric coupling between α-rings of the 20S proteasome
a Kinetics of LFP peptide degradation by symmetric (blue) and asymmetric (yellow) 20S CP alone and with double (black) and single (red) activator PA26PANc. Three (n = 3) biologically independent experiments were carried out. The means are used to derive the curve and the error bars represent standard deviation. b Time course of GFP-ssrA degradation by PAN-αWTββαK66A and PAN-αWTββαWT-PAN, analyzed by Coomassie Blue stained SDS–PAGE. The experiment was repeated two times; each produced similar results. c Time course of GFP-ssrA degradation by PAN-αWTββαK66A and PAN-αWTββαWT-PAN, analyzed by western blotting with anti-GFP antibody. The MW reference proteins were prestained before applying anti-GFP antibody. The experiment was repeated two times; each produced similar results.
