Table 1 Summarization of the kcat (1/min), KM (μM), and Vmax (μM/min) of LFP peptide degradation by symmetric and asymmetric 20S CP without and with activators.
From: Allosteric coupling between α-rings of the 20S proteasome
20S CP | αwtββαwt | αwtββαwt | αwtββαK66A | αwtββαK66A |
|---|---|---|---|---|
activator | PA26PANc | PA26PANc | ||
Kcat (1/min) | 4.13 ± 0.37 | 12.44 ± 1.19 | 4.14 ± 0.28 | 14.40 ± 1.10 |
KM (μM) | 5.80 ± 1.53 | 11.17 ± 2.39 | 5.96 ± 0.96 | 19.27 ± 2.65 |
Vmax (μM/min) | 66.29 ± 5.59 | 199.1 ± 19.1 | 82.28 ± 4.51 | 230.5 ± 17.5 |
