Table 2 Table summarizes the kcat (1/min), KM (μM), and Vmax (μM/min) kinetic parameters of peptidase activity of symmetric and asymmetric 20S CP without and with PA26 activator.
From: Allosteric coupling between α-rings of the 20S proteasome
20S CP | αwtββαwt | αwtββαwt | αwtββαK66A | αwtββαK66A |
|---|---|---|---|---|
activator | PA26 | PA26 | ||
Kcat (1/min) | 7.89 ± 0.38 | 50.34 ± 4.91 | 5.82 ± 0.46 | 25.54 ± 2.61 |
KM (μM) | 4.14 ± 0.65 | 37.31 ± 5.24 | 7.06 ± 1.41 | 24.48 ± 4.05 |
Vmax (μM/min) | 126.2 ± 6.1 | 805.4 ± 78.6 | 93.2 ± 7.3 | 408.6 ± 41.7 |
