Fig. 10: Optimization of image quality and radiation dose.
From: In vivo localization of chronically implanted electrodes and optic fibers in mice
a Top, acquisition settings for CT; middle, corresponding image of a tetrode drive implant (grayscale images; for silicon probe and optical fiber implants, see Supplementary Fig. 9); bottom, radiation effects and measured doses (n = 3 mice in each group; n = 15 mice in total). Error bars show standard deviation from the mean; markers show individual data points. The yellow arrow shows a bent single tetrode in the magnified image, indicating the spatial resolution of the images. Red arrows show artifacts caused by the metal parts of the microdrive, more apparent for lower exposure time and number of projections. b Average normalized body weight of mice (n = 3 mice in each group, n = 9 mice in total) exposed to two CT scans separated by a 1-week pause, mimicking the protocol used for the localization procedure. The first scan was performed with setting (v), and the settings for the second scan are indicated by the color code adopted from panel a. Error shades show standard deviation from the mean. c Performance of scanned animals (n = 7 mice, settings (v) and (iii), blue) in a standard five-choice serial reaction time task did not differ (one-tailed Mann–Whitney U test for each day, P > 0.1) from that of the control population (n = 7 mice, gray). Error shades show mean ± standard deviation.
