Fig. 2: CT-based implant localization.
From: In vivo localization of chronically implanted electrodes and optic fibers in mice
a Raw preoperative CT image. Canonical 3D axes used in brain atlas coordinate systems (green, pink, and cyan arrow) are not aligned with the coordinate system of the CT image. b The preoperative CT image was aligned to the atlas coordinate system by leveling the Bregma (blue arrows) with the Lambda (yellow arrows) in the sagittal plane (blue dashed lines) and symmetrical bone structures in the coronal and horizontal planes (yellow dashed lines). c After the implantation of an eight-tetrode microdrive, postoperative CT imaging was performed, and the scan was co-registered with the preoperative CT image. The skull was covered with a radiopaque dental adhesive (red arrow), which hid Bregma and Lambda. The implant was segmented (blue area) with an intensity threshold applied on a whole-brain region of interest (ROI). CT images are shown in grayscale. Images in each row from left to right, maximum intensity projection, sagittal slice, horizontal slice, and coronal slice. Yellow numbers refer to the corresponding mediolateral, dorsoventral, and anteroposterior stereotaxic coordinates in mm from Bregma.
