Fig. 8: Quantification of localization accuracy.
From: In vivo localization of chronically implanted electrodes and optic fibers in mice
a Anteroposterior (AP), mediolateral (ML), and dorsoventral (DV) offset from the target coordinate for an example animal. Teal, CT-based coordinate system alignment; blue, CT–MRI fusion-based atlas co-registration; salmon, histological track reconstruction. Ellipses on the left represent the area covered by the tip of the implant in the horizontal plane. b Mean absolute difference of tip coordinates in three directions with respect to histology. CT–MRI fusion-based localization was significantly closer to histology results in the AP direction. **P < 0.01; P = 0.0024; two-tailed Wilcoxon signed-rank test. Individual data points (n = 12 implants) are shown with different symbols representing target areas. c Mean distance from histology based on the overlap of implanted areas for tetrode implants. CT–MRI fusion-based localization was significantly closer to histology results in the AP direction. **P < 0.01; P = 0.0078; two-tailed Wilcoxon signed-rank test. Individual data points (n = 9 implants) are shown with different symbols representing target areas as in panel b. d Coronal and sagittal offset from the planned direction of the trajectory for the same example animal as in panel a. Implant directions were quantified based on the CT images. Histology only provides coronal offset measures. e Mean absolute difference of CT-based coronal angle compared to histology. Individual data points (n = 12 implants) are shown with different symbols representing target areas as in panel b. f Mean offset from the target of the centroid of the localized areas with CT–MRI fusion-based localization. Individual data points (n = 12 implants) are shown with different symbols representing target areas as in panel b. g Mean offset from the coronal (n = 12 implants) and sagittal (n = 11 implants) target direction with CT-based localization. Anterior, lateral, and ventral directions were defined as the positive directions. Error bars and gray shadings in panel g show mean ± standard deviation.
