Fig. 1: Whole CRESS virus genomes are integrated in Entamoeba genomes.
From: Entamoeba and Giardia parasites implicated as hosts of CRESS viruses
a Cropped nucleotide alignment between Entamoeba dispar contig (AANV02000527.1) containing a complete virus integration and the genome of Entamoeba-associated CRESS DNA virus 1, isolate 84-AMS-03 (accession MT293413.1); also see Supplementary Fig. 2. Coloured vertical bars denote single nucleotide variations between the sequences (adenine = green, guanine = red, thymine = blue, cytosine = orange), with conservation across the alignment displayed below. b Dotplot of BLAT generated nucleotide alignment between endogenous viral elements and flanking sequence from two closely related Entamoeba species (x-axis sequence reverse complemented). c Example of the circular genome organisation of identified CRESS viruses. d Exogenous virus DNA is protected by a viral capsid, as it can be PCR-amplified after filtration and treatment with DNase (one independent experiment).
