Fig. 5: The neuronal surface is altered during homeostatic plasticity.

a Upper panel: Example electrophysiological recording from an untreated cell. Insert shows magnification of neighboring fast (EPSC) and slow (IPSC) synaptic events. The fast EPSC is further magnified for clarity. Lower panel: Example recording trace from a TTX treated cell. Insert shows magnification of a synaptic burst event. b Plot shows averaged fast EPSC amplitudes for each cell (triangles) and their averages (mean ± s.e.m) for untreated (n = 16), BIC (n = 27), and TTX (n = 29) treated cells. Untreated vs. BIC p = 0.086, Untreated vs. TTX, p = 0.043. Mann–Whitney test. ***p < 0.001 c Plot shows the frequency of synaptic bursts for each cell (triangles) and their averages (mean ± s.e.m) for untreated (n = 38), BIC (n = 55), and TTX (n = 56) treated cells. Untreated vs. BIC, p = 0.17, untreated vs. TTX, p < 0.001. Mann–Whitney test. ***p < 0.001 d Scatterplot comparing surface abundance changes during synaptic upscaling and downscaling. Significantly regulated proteins (fold-change > 1.5 and p < 0.05) are indicated by color. e Scatterplot comparing surface abundance changes during synaptic upscaling and downscaling as shown in d with protein groups of interest indicated. f Scatterplot showing abundance change (scaling vs. control) of synaptic upscaling comparing surface (x-axis) and total (y-axis) pools. Significantly regulated proteins (fold-change > 1.5 and p < 0.05) are indicated by color. g Scatterplot showing abundance change (scaling vs. control) of synaptic downscaling comparing surface (x-axis) and total (y-axis) pools. Significantly regulated proteins (fold-change > 1.5 and p < 0.05) are indicated by color. Source data are provided as a Source Data file.