Fig. 6: Surface trafficking of diverse cargo during cLTP.

a cLTP paradigm b The plot shows averaged fast EPSC amplitudes for each cell (triangles) and their averages (mean ± s.e.m) for control (untreated, n = 45) and cLTP (glycine treated, n = 38) cells. Mann–Whitney test. c–f Boxplots of distributions of log2 surface abundances for replicates of cLTP (n = 12) and control (n = 12). Boxes indicate median and percentiles (25th and 75th). g Volcano plot of statistical significance (y-axis) and surface abundance change (x-axis). Horizontal line is located at an adjusted p-value of 0.05. h Proteins with significantly different surface abundances (fold-change > 1.5 and p < 0.05) after cLTP grouped into categories. Shape size indicates scaled −log10 adjusted p-value. Border color indicates fold-change directionality (green > 0, purple < 0). Edges represent string confidence > 0.7. i Representative images of analysed primary dendrites before and after cLTP induction. Bars 5 µm. j Quantification of mean fluorescent intensity of antibody signal on the primary dendrite surface using antibodies against GluA: n = 63, Bai1: n = 42, Adcy3: n = 28 (CTRL) n = 22 (cLTP) cells/group. Means + 95% CI presented. Two-way T-test (Adcy3 + Bai1) or Mann–Whitney test (GluA). *p < 0.05, **p < 0.01, ****p < 0.0001. Source data are provided as a Source Data file.