Fig. 1: Structural characterization of hPolμΔ2 engaging a complementary DSB substrate.
From: Structural snapshots of human DNA polymerase μ engaged on a DNA double-strand break
a DNA substrate crystallized with hPolμΔ2 and nonhydrolyzable dUMPNPP. Template strand discontinuity is marked (dashed red line). b Pre-catalytic quaternary DSB (protein, dark green; DNA and dUMPNPP colored as in a; template break site, red) The ordered ends of Loop1 are indicated (Gln364/Ala384). c hPolμΔ2 pre-catalytic quaternary complex active site. 2Fo–Fc electron density (gray mesh) contoured at 1σ. Ionic interactions (Mg2+ ion, purple; water molecules, red) are indicated by black bars. Interatomic distance between 3′-OH and α-phosphate (dashed cyan line) was measured in PyMOL (Schrödinger). d Zoomed-in view of the hPolμΔ2 active center, with arrows indicating putative electron movements during reaction progression.
