Fig. 2: Comparison of hPolμΔ2 interactions with SSB or complementary DSB substrates.
From: Structural snapshots of human DNA polymerase μ engaged on a DNA double-strand break
Superposition of hPolμΔ2 pre-catalytic complexes bound to a DSB (colored as in 1a) or a 1nt-gapped SSB (gray, PDB ID code 4M0421). Interactions involving downstream duplex a, template strand b, and upstream primer strand c near the break site are diagrammed (Table 2). Putative hydrogen bonds in DSB and SSB complexes drawn as dashed lines (black or gray, respectively). Ionic interactions (solid lines) are color-coordinated with metal identity. Red and blue arrows indicate template backbone break and bend, respectively. Arg449 and Arg175 sidechains in a are partially disordered and only the ordered regions are included in the model.
