Fig. 4: Qualitative assessment of the role of His208 in NHEJ proficiency.
From: Structural snapshots of human DNA polymerase μ engaged on a DNA double-strand break
Activity of full-length wildtype hPolμ versus the Polμ H208A mutant in in vitro NHEJ concatamerization assay using complementary (top) or noncomplementary (bottom) DSB ends, as depicted in cartoons beside each panel. Downstream primer is 5′-phosphorylated (black circle). Pols λ and β are included for reference, as Pol β shows no detectable activity for either substrate, whereas Pol λ is active only on the partly complementary DSB. Source data are provided as a Source Data file.
