Fig. 6: Comparison of the hPolμΔ2 complementary DSB and mouse TdT crystal structures.
From: Structural snapshots of human DNA polymerase μ engaged on a DNA double-strand break
Superposition of hPolμΔ2 complementary DSB (colored as in Fig. 1a–b) with mouse TdT DSB synapse with a nicked complementary DSB substrate (khaki, PDB ID code 5D4623) or a c single-nucleotide complementary DSB substrate otherwise lacking an upstream template (light blue, PDB ID code 4QZ822), with zoomed-in view of superimposed DNA substrates b, d. Loop1 residues from TdT are drawn in cartoon (brown and blue, respectively). e Superimposed DNA substrates from the TdT crystal structures, colored as in c, d to highlight the “wedged” upstream residues (dashed yellow circle). Red arrow indicates the location of the broken template strand. The magenta and orange asterisks indicate the positions of the “wedged” upstream residues and the hPolμΔ2 Loop2 deletion, respectively.
