Fig. 1: Myocardial energy metabolism dysfunction and PGC1α downregulation are observed in the heart of CKD mice.
From: IRF1-mediated downregulation of PGC1α contributes to cardiorenal syndrome type 4
a GO enrichment of the significantly changed cellular components of the heart from three pairs of sham and CKD mice. b KEGG pathway classification of differentially expressed genes (DEG) of the heart in sham and CKD mice. c Representative images of transmission electron microscope (TEM) observation of mitochondria of the heart from sham and CKD mice (n = 3 mice per group). Scale bar, 2 μm. Red arrows point to mitochondria. d Heatmap of DEG (mainly PGC1α and its target genes) of mice in a. The color intensity represents row Z-score. Red color indicates highly expressed, while blue indicates lowly expressed. e–g Relative mRNA expression of FAO genes (e), OXPHO genes (f), and PGC1α and its target genes (g) of the heart from sham and CKD mice. h Western blot analysis of PGC1α expression of the heart from sham and CKD mice. Microarray data were obtained from three mice per group. Data in a, b are shown as −LogP and were analyzed by Fisher’s exact test, adjusted by using Benjamini–Hochberg. Data in e–h are shown as mean ± SD and were analyzed by a two-tailed unpaired t-test (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001.
