Fig. 3: Expression of DICER1 and Alu in human diabetic adipocytes and skeletal myocytes. | Nature Communications

Fig. 3: Expression of DICER1 and Alu in human diabetic adipocytes and skeletal myocytes.

From: Repurposing anti-inflammasome NRTIs for improving insulin sensitivity and reducing type 2 diabetes development

Fig. 3

The top panels show the results of western blotting of extracts of proteins from human adipocytes a and human myocytes b isolated from nondiabetic and diabetic persons. Immunoreactive bands corresponding to DICER1 and beta-actin (β-actin) are shown. The bottom panels show bar graphs of the densitometric analyses of the DICER1 western blots in the top panels that have been normalized to β-actin abundance and to the nondiabetic group data. *P = 0.04 (diabetic versus nondiabetic adipocytes), *P = 0.046 (diabetic versus nondiabetic myocytes), two-tailed unpaired Student t test. The top panels show the results of northern blotting of total RNA extracts from human adipocytes c and human myocytes d isolated from nondiabetic and diabetic persons. Hybridization bands corresponding to Alu RNA and 5.8S ribosomal RNA (5.8S rRNA) are shown. The bottom panels show bar graphs of the densitometric analyses of the Alu northern blots in the top panels that have been normalized to 5.8S rRNA abundance and to the nondiabetic group data. *P = 0.04 (diabetic versus nondiabetic adipocytes), *P = 0.03 (diabetic versus nondiabetic myocytes), two-tailed unpaired Student t test. Data are reported as mean ± s.e.m. n = 5 samples per group a–d. Source data are provided as a Source Data file.

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