Fig. 5: Expression of DICER1, B2, and insulin sensitivity in high-fat diet-fed mice. | Nature Communications

Fig. 5: Expression of DICER1, B2, and insulin sensitivity in high-fat diet-fed mice.

From: Repurposing anti-inflammasome NRTIs for improving insulin sensitivity and reducing type 2 diabetes development

Fig. 5

a The top three panels show the results of western blotting of extracts of proteins from subcutaneous adipose tissue (SAT), visceral adipose tissue (VAT), and skeletal muscle tissue isolated from mice fed a normal diet (ND) or a high-fat diet (HFD). Immunoreactive bands corresponding to DICER1 and beta-actin (β-actin) are shown. The bottom three panels show bar graphs of the densitometric analyses of the DICER1 western blots in the top panels that have been normalized to β-actin abundance and to the ND group data. n = 10 (ND) or 9 (HFD) samples per group. *P = 0.01 (ND versus HFD in SAT), P < 0.001 (ND versus HFD in VAT), P = 0.01 (ND versus HFD in Skeletal muscle), two-tailed unpaired Student t test. b The top two panels show the results of northern blotting of total RNA extracts from visceral adipose tissue (VAT) and skeletal muscle tissue isolated from mice fed a normal diet (ND) or a high-fat diet (HFD). Hybridization bands corresponding to B2 RNA and 5.8S ribosomal RNA (5.8S rRNA) are shown. The bottom two panels show bar graphs of the densitometric analyses of the B2 northern blots in the top panels that have been normalized to 5.8S rRNA abundance and to the ND group data. n = 9 (ND) or 10 (HFD) samples per group. *P < 0.001 (ND versus HFD in VAT) and P = 0.001 (ND versus HFD in Skeletal muscle), two-tailed unpaired Student t test. Glucose tolerance test (GTT; c) and insulin tolerance test (ITT; d) measurements, and area under the curve (AUC) quantification in mice fed a normal diet, a high-fat diet and treated with phosphate-buffered saline vehicle (HFD + Vehicle), or a high-fat diet and treated with once-daily intraperitoneal administration of lamivudine (70 mg/kg of body weight) (HFD + Lam). Data are reported as mean ± s.e.m. n = 10 (ND), 8 (HFD + Vehicle), or 10 (HFD + Lam) samples per group c. n = 10 (ND), 8 (HFD + Vehicle), or 9 (HFD + Lam) samples per group d. *In GTT, P < 0.001 (HFD + Vehicle versus Normal Diet) and P = 0.002 (HFD + 3TC versus HFD + Vehicle), two-tailed unpaired Student t test. *In ITT, P < 0.001 (HFD + Vehicle versus Normal Diet) and P = 0.003 (HFD + 3TC versus HFD + Vehicle), two-tailed unpaired Student t test. Source data are provided as a Source Data file.

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