Fig. 1: Myeloid Vdr deletion induces hypertension by increasing vascular ROS.

In 8-week-old KODMAC (light-blue bar) or control mice (white bar): a invasive mean arterial BP (n = 3/group), b plasma renin (n = 5/group), distal thoracic aortic assessment of (c) MOMA staining (green, DAPI = blue) (representative of four/group, scale bars: 100 µm), d DHE staining (red, DAPI = blue) in the presence or absence of PEG-superoxide dismutase (representative of four/group, scale bars: 100 µm), e NADPH-stimulated lucigenin-enhanced chemiluminescence (n = 4/group), f increase in mean arterial blood pressure (MAP) with L-NAME (n = 5 Con/4 KOD), and g renal cortical blood flow (n = 8/group). Irradiated KODMAC mice transplanted with BM from KODMAC (KOD→KOD) (light-blue bar) or control (Con→KOD) (yellow bar) and irradiated control mice transplanted with BM from KODMAC (KOD→Con) (dark-blue bar) or control (Con→Con) (white bar). h Noninvasive BP (n = 24–33/group) and i plasma renin (n = 5/group). Data presented as mean ± SEM from (a) mixed model analysis, (b–g) Student’s two-tailed unpaired t test with *P < 0.05, ***P < 0.001 vs. control, and (h, i) Student’s two-tailed unpaired t test with ***P < 0.001 vs. matched donor/recipient control transplant.