Fig. 3: Macrophage ER stress regulates miR-106-5p secretion and BP.
From: Macrophage secretion of miR-106b-5p causes renin-dependent hypertension
In dietary vitamin D-deficient or -sufficient miR-106b−/− or littermates: a noninvasive BP (n = 10/group) and b renin-positive (YFP-labeled Ren1c) JG cells (n = 3/group) after culture with macrophage media. In vitamin D-deficient (light- and dark-blue bars) or -sufficient control mice (white and green bars) transplanted with BM from miR-106b–/– or control mice on matched diet: c noninvasive BP (n = 12 recip/group) and d plasma renin (n = 5 recip/group). In wild-type recipients transplanted with BM from KODMAC/miR-106b−/− (light-blue bar), KODMAC/miR-106+/+ (dark-blue bar), or control Vdr+/+/miR-106+/+ mice (white bar): e noninvasive BP (n = 6/group), f plasma renin (n = 6/group), and g renin-positive JG cells after culture with macrophage media (n = 3/group). In JG cells cocultured with media from vitamin D-deficient peritoneal macrophages treated with (dark-blue bar) or without phenylbutyric acid (PBA) (light-blue bar): h miR-106b-5p abundance and i renin-positive cells (n = 6/group). In JG cells cocultured with media from vitamin D-sufficient peritoneal macrophages treated with (green bar) or without thapsigargin (white bar): j miR-106b-5p abundance and k renin-positive cells (n = 6/group). In Ddit3+/+ or Ddit3−/− mice fed vitamin D-deficient and -sufficient diets: l noninvasive BP (n = 8–11/group), m renin-positive JG cells after coculture with their peritoneal macrophage media (n = 3/group). In vitamin D-deficient (light- and dark-blue bars) or -sufficient control mice (white and green bars) transplanted with BM from Ddit3−/– or control mice on matched diet: n noninvasive BP (n = 6 recip/group) and o plasma renin activity (n = 6 recip/group). Data expressed as mean ± SEM from (a, b, e–g, l, m) one-way ANOVA with Tukey’s post hoc test with ***P < 0.001 vs. all, (c, d, n, o) Student’s two-tailed unpaired t test with *P < 0.05, **P < 0.01, ***P < 0.001 vs. matched donor/recipient diet, and (h–k) Student’s two-tailed unpaired t test with *P < 0.05, **P < 0.01, ***P < 0.001 vs. vitamin D-deficient or -sufficient macrophage control media.
