Fig. 1: ObLiGaRe Dox Inducible (ODIn) Cas9, an all in one, universal TET ON inducible system.
From: Development of an ObLiGaRe Doxycycline Inducible Cas9 system for pre-clinical cancer drug discovery
ObLiGaRe Dox-inducible (ODIn) Cas9, an all-in-one, universal Tet-On inducible system a human and mouse cell lines sourced from brain (N2A), lung (A549), colon (HCT116), ovarian tissue (OVCAR8), liver (HepG2), kidney (HEK293), and human-induced pluripotent stem cells (iPSC) engineered using b the ODInCas9 system targeting the AAVS1 or ROSA26 locus using ZFN-directed ObLiGaRe. c Outlined methodology for rapid cell line generation of ODInCas9 pools and clonal lines encountered with or without exposure to Cas9. Evaluation of GFP expression of d non-induced and e Doxycycline Inducible ODInCas9 cell lines. f Quantification of mismatch endonuclease assay estimating indel formation post sgRNAs transfection of induced ODInCas9 cell lines targeting; MCT1 in OVCAR8, Tpr53 in N2a, and GFAP in HCT116, HEK293, HepG2, A549, and iPSC (n = 7 biological independent cell lines). Scale bar: 300 μm.
