Fig. 1: Structure of the U2AF1 complex bound to 3′ splice site RNA.
From: Elucidation of the aberrant 3′ splice site selection by cancer-associated mutations on the U2AF1
a Sequence elements required for splicing. Boxes indicate exons; Y pyrimidine, R purine, N any nucleotide, 5′SS 5′ splice site, BPS branch-point sequence, PPT polypyrimidine tract, 3′SS 3′ splice site. The dotted line indicates the exon boundary at the 3′ splice site, and numbers indicate the distance from the boundary. b Crystal structure of U2AF1 complexed with RNA, 5′-UAGGU. The N-terminal zinc finger (2-43, ZF1), U2AF homology motif (UHM, 44-141), C-terminal zinc finger (ZF2, 143-170), U2AF2 fragment (105–159), and RNA are colored blue, yellow, green, gray, and magenta, respectively. Inset: close-up view of the RNA-binding region in U2AF1. RNA is shown in stick representation colored with orange.
