Table 2 Data collection and refinement statistics.
From: Elucidation of the aberrant 3′ splice site selection by cancer-associated mutations on the U2AF1
WT-UUAGGU (PDB: 7C06) | WT-UAAGGU (PDB: 7C07) | S34Y-UUAGGU (PDB: 7C08) | |
|---|---|---|---|
Data collection | |||
Space group | P21 | P21 | P21 |
Cell dimensions | |||
a, b, c (Å) | 93.8, 255.1, 94.1 | 94.5, 257.0, 94.6 | 94.5, 259.0, 94.8 |
α, β, γ (°) | 90, 101.1, 90 | 90, 100.8, 90 | 90, 100.7, 90 |
Resolution (Å) | 48.86–3.02 (3.08–3.02)* | 48.17–3.20 (3.27–3.20)* | 48.40–3.35 (3.43–3.35)* |
Rmerge | 0.113 (1.040)* | 0.117 (0.802)* | 0.230 (1.158)* |
Rpim | 0.046 (0.434)* | 0.058 (0.404)* | 0.094 (0.466)* |
CC1/2 | 0.997 (0.727)* | 0.996 (0.638)* | 0.971 (0.742)* |
I / σI | 17.0 (2.1)* | 10.2 (2.1)* | 8.3 (2.2)* |
Completeness (%) | 100.0 (100.0)* | 100.0 (100.0)* | 100.0 (100.0)* |
Redundancy | 7.1 (6.7)* | 4.9 (4.8)* | 7.0 (7.1)* |
Refinement | |||
Resolution (Å) | 3.02 | 3.20 | 3.35 |
No. of reflections | 84,653 | 72,745 | 64,508 |
Rwork/Rfree | 0.2146/0.2487 | 0.2547/0.2854 | 0.2143/0.2523 |
No. of atoms | |||
Protein | 17,455 | 17,409 | 17,486 |
RNA | 945 | 963 | 945 |
Zn | 18 | 18 | 18 |
B-factors | |||
Protein | 73.2 | 79.8 | 72.3 |
RNA | 81.9 | 92.1 | 80.9 |
Zn | 57.9 | 60.6 | 48.9 |
R.m.s. deviations | |||
Bond lengths (Å) | 0.002 | 0.002 | 0.002 |
Bond angles (°) | 0.511 | 0.447 | 0.500 |
