Fig. 2: CD36-mediated caveolar endocytosis transports FAs into cells.

a–c 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, and then treated with BSA-conjugated PacFA (50 μM) for 20 min, followed by UV crosslinking on ice for 30 min. a, b Cells were subjected to click chemistry using an N₃-Alexa Fluro 488, and immunostained with anti-CD36 antibody. Colocalization of PacFA and CD36 was quantified from 24 cells over three independent experiments and plotted in (b). The value represents mean ± SEM. c Cells were lysed and subjected to click chemistry assay using N₃-biotin. PacFA-labeled proteins were captured with streptavidin beads and subjected to western blot using anti-CD36 and anti-FABP4 antibodies. d WT, Cav1^−/−, Cd36^−/−, and Cav1^−/−;Cd36^−/− SVFs were isolated and differentiated into adipocytes and treated with 100 μM ³H-oleate (specific activity, 2268 dpm/nmol) for 1 h. Lipid fractions were extracted from the cells and subjected to scintillation counting. The radioactive counting was normalized to protein content. Each value represents mean ± SEM obtained from three samples. Two-sided Student’s t test was performed between WT and each of the knockout cells. These experiments were repeated twice. Source data are provided as a Source Data file.