Fig. 2: Mutual exclusivity of T-Plastin and myosin localizations: T-plastin localizes to and regulates the size of lamellipodia.
From: T-Plastin reinforces membrane protrusions to bridge matrix gaps during cell migration
a Localization summary of actin-bundling proteins expressed in endothelial cells (localizations shown in Fig. S2a–c). b Sheets of HUVEC migrating into a scratch stained for F-actin (yellow) and endogenous T-Plastin (magenta), bar 20 µm. c Cells treated with control or T-Plastin siRNA were fixed and stained for F-actin (yellow) and endogenous T-Plastin (magenta), bar 10 µm. d Cells treated with control or T-Plastin siRNA were fixed and stained for F-actin (yellow) and endogenous T-Plastin (magenta), and Arp3 (cyan), bar 10 µm. e Cells stably expressing MYL9-mTurquoise (yellow) were transfected with T-Plastin-mCitrine (magenta) and imaged, bar 20 µm. f Cells expressing F-tractin-mCitrine and MYL9-mTurquoise were treated with control or T-Plastin siRNA and imaged. The ratio of MYL9 over F-tractin is shown as a parula colormap (yellow and blue are 99th (high), 3rd (low) percentiles), bar 10 µm. g Quantification of intensities of MYL9 (magenta) and F-tractin (green) along lines drawn from the leading edge into the lamellum of (n cells for siControl = 9, siT-Plastin = 10, from two independent replicates) as shown in (f). Dots represent the median value for each position, lines are LOWESS spline fits of the data, and dotted vertical lines indicate regions of actin at leading edge and where myosin activity begins.
